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disodium 2-[(E)-{4-[ethyl(3-sulfonatobenzyl)amino]phenyl}{(4E)-4-[ethyl(3-sulfonatobenzyl)iminio]cyclohexa-2,5-dien-1-ylidene}methyl]-5-hydroxybenzenesulfonate

dihydrogen (ethyl)[4-[4-[ethyl(3-sulphonatobenzyl)amino](4-hydroxy-2-sulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene](3-sulphonatobenzyl)ammonium, disodium salt

CAS: 2353-45-9

Molecular Formula: C37H34N2O10S3Na2

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  5. disodium 2-[(E)-{4-[ethyl(3-sulfonatobenzyl)amino]phenyl}{(4E)-4-[ethyl(3-sulfonatobenzyl)iminio]cyclohexa-2,5-dien-1-ylidene}methyl]-5-hydroxybenzenesulfonate

disodium 2-[(E)-{4-[ethyl(3-sulfonatobenzyl)amino]phenyl}{(4E)-4-[ethyl(3-sulfonatobenzyl)iminio]cyclohexa-2,5-dien-1-ylidene}methyl]-5-hydroxybenzenesulfonate - Names and Identifiers

Name dihydrogen (ethyl)[4-[4-[ethyl(3-sulphonatobenzyl)amino](4-hydroxy-2-sulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene](3-sulphonatobenzyl)ammonium, disodium salt
Synonyms C.I. 42053
Food Green 3
Fast Green FCF
C.I. Food Green 3
disodium 2-[(E)-{4-[ethyl(3-sulfonatobenzyl)amino]phenyl}{(4E)-4-[ethyl(3-sulfonatobenzyl)iminio]cyclohexa-2,5-dien-1-ylidene}methyl]-5-hydroxybenzenesulfonate
dihydrogen (ethyl)[4-[4-[ethyl(3-sulphonatobenzyl)amino](4-hydroxy-2-sulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene](3-sulphonatobenzyl)ammonium, disodium salt
sodium 3-({[(1E,4E)-4-({4-[(4-ethoxyphenyl)amino]phenyl}{4-[ethyl(3-sulfonatobenzyl)amino]-2-methylphenyl}methylidene)-3-methylcyclohexa-2,5-dien-1-ylidene](ethyl)ammonio}methyl)benzenesulfonate
CAS 2353-45-9
EINECS 219-091-5
InChI InChI=1/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30H,6-8,31-32H2,1-5H3,(H2,51,52,53,54,55,56);/q;+1/p-1

disodium 2-[(E)-{4-[ethyl(3-sulfonatobenzyl)amino]phenyl}{(4E)-4-[ethyl(3-sulfonatobenzyl)iminio]cyclohexa-2,5-dien-1-ylidene}methyl]-5-hydroxybenzenesulfonate - Physico-chemical Properties

Molecular FormulaC37H34N2O10S3Na2
Molar Mass808.85
Melting Point290℃
Water SolubilitySoluble in water, glycerin, glycol and ethanol.
Solubility Easily soluble in water, soluble in ethanol, soluble in concentrated sulfuric acid, dark orange, dark green after dilution, soluble in concentrated hydrochloric acid or concentrated nitric acid, orange, soluble in 10% sodium hydroxide solution, bright blue.
AppearanceForm Powder/Solid, color Red to Red-Brown
PH2.7 (10g/l, H2O, 20℃)
Storage Conditionroom temp
StabilityStable. Incompatible with strong oxidizing agents.
MDLMFCD00013053
Physical and Chemical PropertiesChemical red to brown purple particles or powder with metallic luster. No smell. Easily soluble in water and ethanol, the neutral aqueous solution is blue-green, acidic is green, and alkaline is blue to blue-purple. Dissolved in concentrated sulfuric acid and brown-orange, diluted with water and turned green. Heat resistance, light resistance, good reduction resistance, stable to tartaric acid and citric acid. Weak alkali resistance. Strong hygroscopicity.
UseUse edible green pigment. It is combined with other pigments for confectionery products and refreshing beverages (only for confectionery products in Japan).

disodium 2-[(E)-{4-[ethyl(3-sulfonatobenzyl)amino]phenyl}{(4E)-4-[ethyl(3-sulfonatobenzyl)iminio]cyclohexa-2,5-dien-1-ylidene}methyl]-5-hydroxybenzenesulfonate - Risk and Safety

Hazard SymbolsXn - Harmful
Harmful
Risk CodesR20/21/22 - Harmful by inhalation, in contact with skin and if swallowed.
R40 - Limited evidence of a carcinogenic effect
Safety DescriptionS25 - Avoid contact with eyes.
S37/39 - Wear suitable gloves and eye/face protection
WGK Germany3
RTECSBQ4425000
FLUKA BRAND F CODES3
TSCAYes
HS Code32041900
ToxicityLD50 orally in rats: >2 g/kg (Lu, Lavalle)

disodium 2-[(E)-{4-[ethyl(3-sulfonatobenzyl)amino]phenyl}{(4E)-4-[ethyl(3-sulfonatobenzyl)iminio]cyclohexa-2,5-dien-1-ylidene}methyl]-5-hydroxybenzenesulfonate - Upstream Downstream Industry

Raw MaterialsSodium dichromate dihydrate
p-Hydroxybenzaldehyde
N-Benzyl-N-ethylaniline
Dichromate, Ion chromatography standard solution, Specpure, Cr2O7ˉ2 1000μg|ml
2-Formyl-5-hydroxybenzenesulfonic acid
N-Ethyl-N-benzylaniline-3'-sulfonic acid

disodium 2-[(E)-{4-[ethyl(3-sulfonatobenzyl)amino]phenyl}{(4E)-4-[ethyl(3-sulfonatobenzyl)iminio]cyclohexa-2,5-dien-1-ylidene}methyl]-5-hydroxybenzenesulfonate - Reference

Reference
Show more
1. Zhong Yi Xin He, Hu Ke, Peng Huasheng, et al. Tuber Tissue Structure and Alkaloid Histochemical Localization of Wild Corydalis Rhizoma [J]. Journal of Anhui College of Traditional Chinese Medicine, 2018, 037(005):75-78.
2. Zhao Wenping, Jia Longgang, Lu Fuping, et al. Screening System of Aβ42 Aggregation Inhibitor in Escherichia coli Based on β-lactamase [J]. Food and Fermentation Industry, 2019, 45:17-24.

disodium 2-[(E)-{4-[ethyl(3-sulfonatobenzyl)amino]phenyl}{(4E)-4-[ethyl(3-sulfonatobenzyl)iminio]cyclohexa-2,5-dien-1-ylidene}methyl]-5-hydroxybenzenesulfonate - Introduction

Staining of plant histology and cytology. It is a basic dye used for protein dyeing in IEF, PAGE and SDS-PAGE. Fast green staining covers a wider range of protein concentrations than bright blue R(Brilliant Blue R) and is linear. After electrophoresis, it is more sensitive to fix the protein on the gel for dyeing. Dissolve the protein on the gel with 0.1% fast green in 30% (v/v) ethanol, 10% (v/v) acetic acid or 7% acetic acid for 2 hours and then dedye with 30% (v/v) ethanol, 10% (v/v) acetic acid or 7% acetic acid. The dye gel can be browsed at 625 nm. The detection sensitivity is 30% higher than that of Brilliant Blue R. In addition, Kuai Green is also used for sulfite determination, making bacterial culture medium to identify Escherichia coli and other lactose fermentation bacteria, and culture and isolate typhoid bacilli in feces.
Last Update：2022-10-16 17:15:09

disodium 2-[(E)-{4-[ethyl(3-sulfonatobenzyl)amino]phenyl}{(4E)-4-[ethyl(3-sulfonatobenzyl)iminio]cyclohexa-2,5-dien-1-ylidene}methyl]-5-hydroxybenzenesulfonate - Nature

Solubility H2O: soluble50mg/mL, clear, colorless to faintly yellow
color index 42053
Odor Odorless
maximum wavelength (& lambda;max) 532 nm
Sensitivity Hygroscopic
Decomposition 290 °C
Merck 14,3941
BRN 5718212
biological applications Drug delivery and tissue engineering; photodynamic therapy; dental materials; treating cancer,diabetes; wound dressing materials
(IARC) Carcinogen Classification 3 (Vol. 16, Sup 7) 1987
EPA chemical information C. I. Food Green 3, disodium salt (2353-45-9)
Last Update：2024-04-09 19:04:56

disodium 2-[(E)-{4-[ethyl(3-sulfonatobenzyl)amino]phenyl}{(4E)-4-[ethyl(3-sulfonatobenzyl)iminio]cyclohexa-2,5-dien-1-ylidene}methyl]-5-hydroxybenzenesulfonate - Uses and synthesis methods

application

solid green FCF(fastgreenFCF, molecular formula is C37H34N2O10S3Na2) is a biological stain, which can be used as a qualitative labeling reagent for proteins.

Artificial dyes

namely aniline dyes or coal tar dyes, there are many types and extremely wide applications. Its disadvantage is that it is easy to fade after sunlight, and aniline blue, bright green, methyl green, etc. are more likely to fade. Pay attention to the pH in the production, and avoid direct sunlight, and it can not fade for several years.

Fast green (English Fast green) is an acid dye that is soluble in water (solubility is 4%) and alcohol (solubility is 9%). Solid green is a dye agent for dyeing cellulose cell tissue containing pulp, which is widely used in dyeing cells and plant tissues. It is listed as the three most commonly used dyes in plant histology alongside hematoxylin and safranine.

content analysis

measured according to method 2 in the OT-15, the conditions of use are as follows. The sample weight was 1.9~2.0g; The buffer solution was prepared with 15g of sodium hydrogen tartrate. 0.1mol/L TiCl3 per mL is equivalent to 0.04045g of pigment.

Toxicity

ADl 0~25 mg/kg(FAO/WHO,2001); LD50>2 g/kg (rat, oral).

use limited

FAO/WHO,1984(mg/kg): apple sauce, pear, canned processed peas, jam and jelly, 200; Cold drinks, 100 (the total amount of pigment in the final product can reach 300); Orange peel jelly 100; Pickled cucumber 300.

CFR,& sect;74.203(2000): limited to GMP.

use

Used as food, medicine and cosmetic pigment. Used in combination with other food pigments in foods such as cakes and cool drinks, the usage amount is 1-200,000 parts. According to Japan's "Food Additives Public Agreement", the content of this product should be above 85%.

production method

After being condensed and sulfonated by p-hydroxybenzaldehyde and N-ethyl-N-phenylbenzylamine, it is oxidized into pigment with dichromate, permanganate, or lead peroxide, and then neutralized, salted out, and refined.

Last Update：2024-04-09 21:01:54
disodium 2-[(E)-{4-[ethyl(3-sulfonatobenzyl)amino]phenyl}{(4E)-4-[ethyl(3-sulfonatobenzyl)iminio]cyclohexa-2,5-dien-1-ylidene}methyl]-5-hydroxybenzenesulfonate
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Product Name: dihydrogen (ethyl)[4-[4-[ethyl(3-sulphonatobenzyl)amino](4-hydroxy-2-sulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene](3-sulphonatobenzyl)ammonium, disodium salt Request for quotation
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View History
disodium 2-[(E)-{4-[ethyl(3-sulfonatobenzyl)amino]phenyl}{(4E)-4-[ethyl(3-sulfonatobenzyl)iminio]cyclohexa-2,5-dien-1-ylidene}methyl]-5-hydroxybenzenesulfonate
Raw Materials for disodium 2-[(E)-{4-[ethyl(3-sulfonatobenzyl)amino]phenyl}{(4E)-4-[ethyl(3-sulfonatobenzyl)iminio]cyclohexa-2,5-dien-1-ylidene}methyl]-5-hydroxybenzenesulfonate
Sodium dichromate dihydrate
p-Hydroxybenzaldehyde
N-Benzyl-N-ethylaniline
Dichromate, Ion chromatography standard solution, Specpure, Cr2O7ˉ2 1000μg|ml
2-Formyl-5-hydroxybenzenesulfonic acid
N-Ethyl-N-benzylaniline-3'-sulfonic acid
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